Figure 5

Stellate cells (SC) and liver sinusoidal endothelial cells (LSEC). Following intravenous administration of fluorescently labeled substances, sections were prepared as described in the Methods section for transmission electron microscopy. Ultrathin sections were immunodouble labeled to visualize both FITC-labeled formaldehyde-treated serum albumin (FSA) in LSEC and desmin in SC. Figures B and C are higher magnification of segments of figure A. Cells lining the sinusoids (A) are LSEC as judged by the localization of small gold particles (5 nm, small arrow) in organelles taken as lysosomes (B). The cell containing large fatty vacuoles (FV) and large gold particles (10 nm, large arrow), was judged as a stellate cell (SC) (C). (Scale bars; A: 1 μm, B: 200 nm, C: 500 nm).