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Figure 7 | Comparative Hepatology

Figure 7

From: Severe diabetes and leptin resistance cause differential hepatic and renal transporter expression in mice

Figure 7

Trascription factor Nrf2, and nuclear receptor Ppar-α, Fxr, Pxr, Car and their target genes mRNA expression in livers of C57BKS and db/db mice. Messenger RNA expression of Nrf2, Ppar-α, Fxr, Pxr, Car, Gclc, Cyp4a14, Cyp2b10, Cyp3a11 and Shp was quantified. Total RNA was isolated from livers of adult db/db and C57BKS mice, and mRNA expression was quantified using the branched DNA signal amplification assay. The data plotted as average RLU per 10 μg total RNA ± SEM. Asterisks (*) represent a statistically significant expression difference between db/db mice and C57BKS mice of the same gender (p≤0.05). Number signs (#) represent a statistically significant expression difference between male and female db/db mice or male and female C57BKS mice. Nrf2 and its target gene Gclc display increase in male as well as female db/db mice, as compared to respective C57BKS controls. Similarly, Ppar-α and Cyp4a14 expression also increased in db/db mice. Car expression was increased in male db/db mice, and its target gene Cyp2b10 expression was also increased in male as well as female db/db mice. Fxr expression was unaltered in both male and female db/db mice, however, Shp, a target gene of Fxr, displayed increase in female db/db mice as compared to C57BKS females. Similarly, Pxr expression wasn’t altered, however, it’s target gene Cyp3a11 expression was increased in male db/db mice.

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