Immunohistochemical staining of liver sections for Abcc3 detection. Frozen livers were cut to 5 μm cryosections and fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS). Sections were blocked in goat serum followed by incubation with anti-Abcc3. Sections were washed with PBS and incubated with goat anti-rat IgG conjugated to Alexfluor 488 (green staining) and rhodamine-conjugated phalloidin (red). Sections were then rinsed with PBS/T, PBS, and water, air dried, and then mounted with Prolong Gold containing DAPI (blue staining). All images are displayed as 200X magnification. It was observed that green staining displaying Abcc3 expression was higher in db/db male and female mice as compared to controls.