Figure 6

The effects of TCDD on the stability of vitellogenin (VTG) and estrogen receptor α (ERα) mRNAs. Primary cultured hepatocytes were treated with E2 (10 nM) for 24 hours. The synthesis of RNA was then inhibited using actinomycin D (0.5 mg/ml medium). The cells were incubated for 0 to 24 hours in the presence or absence of TCDD (10 nM). The total RNA was then prepared from samples and 7.5 μg per slot of each sample was applied to nylon membranes. The membranes were sequentially hybridized with the [α-³²P]dCTP labelled ERα and VTG probes. Radioactivity in each slot was quantified using phosphoimager as photo stimulated luminescence (PSL). The PSL values of two independent experiments are shown as mean ± 2SD in the plot diagram.