Figure 2
From: Pit cells exclusively kill P815 tumor cells by the perforin/granzyme pathway
The effect of inhibitory compounds on the killing of P815 cells by hepatic NK cells as determined by DNA fragmentation and 51Cr release. CON, control; EGTA, 5 mmol/L EGTA present during coincubation; DCI, preincubation of hepatic NK cells with 50 micromolar/L DCI for 30 minutes, no DCI present during coincubation; VAD, preincubation of P815 cells with 160 micromolar/L Z-VAD-FMK for 30 minutes, 80 micromolar /L present during coincubation. Values are means of three independent experiments. Error bars, SD. (From reference 8, with permission.)