Precision-cut liver slices in culture as a tool to assess the physiological involvement of Kupffer cells in hepatic metabolism

Table 1 Effect of Kupffer cell inhibition by GdCl₃ on lipid synthesis by PCLS

Acetate equivalent incorporated into lipids (nmol/mg prot.)	Gd-	Gd+
- phospholipids	3.7 é 0.1	6.1 é 0.6*
- triglycerides	2.4 é 0.6	4.0 é 0.8*
- cholesterol	0.8 é 0.2	2.0 é 0.2*

PCLS, prepared from 24 h-fasted rats pretreated with GdCl₃ (Gd+) or NaCl (Gd-) 2 days before the experiment, were incubated in medium containing 2 mM [¹⁴C]-acetate for 3 h. Values are means é S.E.M (n é 4) (*p < 0.05, Student t-test).