Figure 1
From: Signaling role of iron in NF-kappa B activation in hepatic macrophages
(A.) Cultured HM were transfected with a TNF-alpha promoter-lucigerase construct followed by the treatment with Fe2+, Fe3+, Cu1+ or Cu2+. The data were normalized by co-transfected Renilla luciferase activity. Note the Fe2+ induces the promoter activity by 2–3 fold at 10 and 50 micromolar. Cu1+ slightly induces but oxidized metals (Fe3+ and Cu2+) do not. (B.) HM were co-transfected with the promoter-luciferase construct with a vector of super-repressor I-kappa B-alpha (DN-I-kappa B), followed by addition of Fe2+. Note DN-I-kappa B completely blocks iron induced promoter activity.