Figure 6From: Liver sinusoidal endothelial cells represents an important blood clearance system in pigsFluorescence micrographs of cultured liver sinusoidal endothelial cells (LSEC). Cultures were prepared as described in the Methods section. The cultures were fixed in 4% paraformaldehyde, after 6 h of incubation. FITC-labeled formaldehyde-treated serum albumin (FSA) and TRITC-labeled monodisperse polymer particles (MDPP) were administered intravenously prior to isolation of liver cells. Fluorescent microscopy reveals a homogeneous LSEC culture contaminated by a few cells with TRITC-MDPP and lipid containing vacuoles. The green fluorescence from endocytosed FITC-FSA demonstrates that most cells are LSEC, and that the probe is localized in cytoplasmic vacuoles (A), whereas the yellow fluorescence from phagocytosed TRITC-MDPP identifies Kupffer cells (arrows) (B). Autofluorescence from vitamin A identifies stellate cells (arrows) (C). (Scale bars; 20 μm).Back to article page