Figure 5

NAC and IFN treatment induce apoptosis in HCC cells. Cells were treated with IFN 2.5x10⁴ U/mL and/or NAC 10 mM for the indicated time periods. Fluorescence microscopy of HepG2 and Huh7 cells stained with annexin and PI. FITC-labelled Annexin V show apoptotic cells (green) and PI stained cells (red) indicate necrosis or late apoptosis. DAPI stained nuclei (blue). Magnification used was 60x. Bar, 25μm.