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Figure 1 | Comparative Hepatology

Figure 1

From: MHC class I expression protects rat colon carcinoma cells from hepatic natural killer cell-mediated apoptosis and cytolysis, by blocking the perforin/granzyme pathway

Figure 1

Effect of anti-MHC I mAb OX18 on hepatic NK cell-mediated CC531s cell cytolysis (A, 51Cr release) and apoptosis (B, DNA fragmentation). (A), 51Cr-labeled CC531s cells were incubated at an E:T ratio of 10:1 with freshly isolated hepatic NK cells for 18 hours. Cytolysis was measured in a 51Cr-release assay. (B), [3H]-TdR labeled CC531s cells were incubated at an E:T ratio of 10:1 with hepatic NK cells for 3 hours. Apoptosis was measured in a quantitative DNA fragmentation assay. ConAb, control antibody. *p < 0.05, **p < 0.01 vs. the treatment with medium only (LSD test).

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Comparative Hepatology

ISSN: 1476-5926